Details
TECHNICAL INFORMATION
Other Names: CT99021, 6-((2-((4-(2,4-Dichlorophenyl)-5-(4-methyl-1H-imidazol-2-yl)pyrimidin-2-yl)amino)ethyl)amino)nicotinonitrile
Chemical Formula: C22H18Cl2N8
CAS Number: 252917-06-09
Molecular Weight: 465.34
Purity: >98% by HPLC
IC50= 6.7nM
Appearance: Off White Solid
Solubility: DMSO (100mM)
STORAGE AND HANDLING
Storage: Store at 4°C and protected from light. Following reconstitution, store aliquots at -20°C.
Stability: Stock solutions stable at -20°C for up to 2 years.
Shipping Conditions: Shipped at room temperature.
PRODUCT USE
Soluble in DMSO (100mM). For a 10 mM solution, reconstitute by adding 430µl of DMSO to the entire contents of the vial. For most cells, the maximum tolerance to DMSO is <0.5%. Incubate in a 37°C water bath for 5 minutes if a precipitate is observed.
When used in combination with PD0325901, CHIR99021 has been shown to sustain ES cell self-renewal.
